| 24 cellulose-decomposing bacteria were isolated from soil and rubbish compost by selective liquid medium with xinhua filter paper as the sole carbon source. We got six colonies surrounded by some distinct zones of clearing in cellulose-congo red ag-ar medium. By measuring the enzyme activity of liquid culture filtrates, we got a strain with higher cell-ulase activity.It was named ST-3 and identified as Cellomonas.The fermentation conditions in shaking flasks were investigated. The highest level of cellulose activity was induced in the mendium containing wheat bran.The efficiency of organic nitrogen sources was better than that of inorganic nitrogen sources, while beef extract was the best organic nitrogen source. The components of the medium were optimized by the method of orthogonal experiment, when ST-3 was cultured under the optimum conditions, the optimal time for CMCase and FPA was 22h and 16h.Properties of cellulose from ST-3 were stuied. The results showed that the optimum temperature and pH for CMCase was 50℃ and 4.0, respectively; FPA was 55℃ and 5. 0. For CMCase and FPA, Co2+ could significantlypromote enzyme activity, while Hg2+, Ag2+, Mn2+ could restrain enzyme activity.The experiment that decomposing the cellulose waste and rubbish compost by ST-3 showed that the soluble reducing sugar could be increased significantly in the liquid medium with corn straw, wheat straw, rice straw,wheat bran and edible mushroom waste respectivly as the sole carbon source. It proved that ST-3 could decompose them, especially for corn straw and wheat bran.To some degree ST-3 could decompose rubbish compost. It is impotent to solve the environment pollution and energy crisis. Therefore, ST-3 has the utilization value to some extent.
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