Isolation And Identification Of Bacteria Degrading Cellulose From Rumen And Optimization Of Product Cellulase Conditions

Cattle and sheep rumen contains a large number of microorganisms,usually include 1010 bacteria and 4×106 protozoa per mL,also can see a large number of yeast and phage.It contains a large number of microorganisms that can degrade cellulose,what can get microorganisms that ecrete cellulase after the filter,separation and purification.After optimizat the microbial enzyme production conditions,identified.A strain is obtained,which by screening high cellulose enzyme activity of bacteria,named Y4,common bacteria cultivation basal medium under the common condition of 48 hour,measuring cellulase activity is 21 U/mL.Observe Y4 colony,are milky yellow in color,irregular shape,has fold in surface,fold down is transparent sticky substance,comprehensive physiological biochemical characteristic and 16S rDNA identification result,recognized Y4 as bacillus subtilis.Using single factor experiment method on enzyme fermentation conditions and fermentation medium optimization fermentation conditions of 40 ? as fermentation temperature,initial pH 6.0,the cradle of revolution after 80 rpm/min.fermentation medium optimization for glucose 2%,yeast extract 0.8%,NaCl 0.5%,KH2PO4 0.1%,K2HPO4 0.3%,MgS04 0.05%.And determine the fermentation curve,Y4 during fermentation for 48 hour to get the highest cellulase activity,40 U/mL,1.9 times before optimization.Bacillus subtilis that secrete cellulase can directly as probiotics to join predatory animal feed,also can produce cellulase preparation,application in food,textile,feed,brewing,oil exploration,chinese medicine ingredient extraction,and many other fields.