Investigation Of Spectrophotometry In Analytical Chemistry

Analytical Chemistry is a science that investigates the principles and technologies relating to component's identifying and content determining. Spectrophotometry is a available means of analytical chemistry using of metallurgy, terra, biology, medicine, pharmacy and agriculture etc. Spectrophotometry measuring system is a way that analyses consistency and structure of some substance in liquor. The design and measuring course's control in the measuring system of ultraviolet-visible spectrophotometry controlled by computer was investigated in the paper.Firstly, it narrated the purpose and significance, investigated actuality and development's trend of spectrophotometry and put forward the task and aim. Secondly, the second part of the paper investigated correlative theory of spectrophotometry measuring. From the third part to the fifth part of the paper, design process of ultraviolet-visible spectrophotometer system was discussed detailedly. After the function and cost of several schemes were contrasted, the design scheme used by computer and measuring block outside computer was confirmed. The pivotal problems of task were discussed and solutions were found. The hardware circuit of data and control signals collection in measuring system was designed. The software of data's processing and measuring control was programme and the measuring process was design. The means of data processing was expatiated and experimentation results were analysed. Available arithmetic of automatic searching absorbency peak and vale by measuring scan spectrum used of ultraviolet-visible spectrophotometer was given. Searching means of multi-peak and multi-vale in sample liquid was solved. This means can overcome effectively almost parts of disturb and avoid limitedly arithmetic and memory beyond range. The arithmetic of one unit linearity return can obtain standard working curve of sample liquid and pertinency coefficient was calculated to estimate standard working curve's pertinency. Finally, the conclusion was given and the investigation's direction was pointed.The task was a actual collaboration item. Every part design of ultraviolet-visible spectrophotometry measuring system was completed and a lot of experimentations were done by it. The experimentation's results and measuring curves were given.