Study On Extraction And Purification Technology And Characteristics Of Mulberry Red Pigment

Mulberry is named mulberry Chinese date, mulberry fruit, mulberry seed. It is the ripe fruit of Moras alba L. Morus alba L are planted in most provinces and areas of China. Mulberry red pigment is a kind of anthocyanin pigment, which can dissolve in water. It shows blue in lye; It shows deep purple and can stably exist in the acidic conditions. It is reported that mulberry red pigment have various nutritional function .So mulberry red pigment as a kind of nature pigment not only act as a coloring agent but possess functional effect in some degree.The test used mulberry residue as material. The optimal parameters of extracting mulberry red pigment by solvent, microwave and ultrasonic wave, the purification parameters of pigment by LSA-21 macroporous resin, the stability and antioxidation in vitro of pigment have been studied in this paper. The above studies can provide references for application and exploration of mulberry resources.The main results showed:1. The optimal parameters of solvent extraction from mulberry residue were as following: solvent is 80% ethanol with 0.01% HCl, material/solvent ratio 1:20, extracting 1.0 h at 20℃, for one time. The extracting rate reached 93.07%.2. The optimal parameters by microwave extraction were as following: solvent is 60% ethanol with 0.01% HCl, microwave firepower medium, extracting 30s, material/solvent ratio 1:15, for two times. The extracting rate reached 95.83%.3. The optimal parameters by ultrasonic extraction were as following: solvent is 60% ethanol with 0.01% HCl, ultrasonic power 200W, extracting lOmin, material/solvent ratio 1:15, for one time. The extracting rate reached 97.76%.4. LSA-21 macroporous resin had good performance of adsorption ability and de-adsorption to mulberry red pigment. The optimal parameters of purification to mulberry red pigment were as following: the best adsorption conditions were concentration of feed 0.425Abs, feeding rate 3.5 BV/h and pH value 1.57; The best watering elution conditions were: elute rate 1.5BV/h, elute volume 1.96BV and eluting 1.4h; The best elution conditions were 80% alcohol, elute rate 1.0BV/h and alcohol volume 2.5BV.5. The stability test of mulberry red pigment showed: mulberry red pigment had inferior stability to pH, ray, heat, oxidizer and reducer; Antiseptic, glucose, fructose and Zn2+, Ca2+, Mg2+, Ba2+, Na+, K+ had little influence on stability of mulberry red...