Extraction And Antioxidant Ability Of Chlorogenic Acid From Flos Lonicerae

Flos Lonicerae, widely spread in China, was among the list of stuffs, which were both medicine and food, announced by the China Ministry of Health, and contained many kinds of bioactive substances.In this paper, the optimum extracting condition of chlorogenic acid from Flos Lonicerae and the antioxidant ability of the extract were studied. Its findings provide the scientific foundation for rational utilization of Flos Lonicerae.Optimization of the extracting method of chlorogenic acid from Flos Lonicerae was studied by several different methods. Quantities and qualitative analysis of chlorogenic acid in Flos Lonicera were developed by Ultraviolet and Visible Spect rophotometric (UV) and High Performance Liquid Chromatographic (HPLC). Detect the bacteriostatic action against pathogenic bacteria in diets, the capacity of exhibiting DPPH·and reducing Fe³⁺.Through the single-factor experiments, the optimum extracting condition was determined. It was 70% ethanol soaking, in the condition that the soaking rate(solution to Flos Lonicerae) was 10:1(v/w), soaking temperature, 80℃, soaking time 90minutes, the extracting rate(extract to solution) 90.38%. It was refined processing condition : D-301 resin, the velocity of absorbed solution is 2 mL/min;it was desorbed by 15% alcohol at 30℃. Chlorogenic acid extraction showed that its tiptop absorb-apex was 324nm.The results suggested that chlorogenic acid has a strong antibacterial action, a superior capacity of exhibiting DPPH·and reducing Fe³⁺.Finally, the physical and chemical attribute of chlorogenic acid was summarized. Chlorogenic acid was a substance stable to light and heat. Then, the relation between the structure of chlorogenic acid and antioxidant ability and its mechanism was analyzed.In this paper we developed a health food product —fast dissolving tabletswith Flos Lonicerae.