Isolation And Identification Of A Marine Polyphosphate-accumulating Bacterium YSR-3 And Research On Its Intracellular Granules

Strain YSR-3, a polyphosphate-accumulating bacterium, was isolatedfrom the Yellow Sea. It can move and its cell is rod-shape, 3.5μm×1μm, Gramnegative, aerobic. It can accumulate polyphosphate in cells and form metachromaticgranules. Based on the 16S rDNA analysis, Strain YSR-3 belongs to genusHalomonas, γ- Proteobacteria. It has some traits in common to Halomonas, but itpossesses its own special characters unlike known species of Halomonas. It issusceptible to some antibiotics, such as Kn and Cl. It has amylase, but denitrificationis negative. It can use glucose as sole carbon source and energy source, however, cannot degrade chitin. It can be cultivated well under these conditions: 3% NaCl,24℃,180rpm, initial pH 6.5.We also did research the ingredients and the shapingconditions of intracellular granules of strain YSR-3. Strain YSR-3 not only belongs to genus Halomonas, but also can accumulatepolyphosphate in cells. This trait is not reported in other strains of the genusHalomonas yet, and this special trait may be important in the research of thephosphorus circulation in the marine. Strain YSR-3 can be valued in the process of thebiological phosphorus removal of seawater. Through the study of the formationprocess of the intracellular phosphorus granules of strain YSR-3,we can providetheoretical basis for the biological phosphorus removal and research material forbiomineralization.