Study On Capillary Electrophoresis And Capillary Electrophoresis-Mass Spectrometry In Analysis Of Complex Matrices

High performance Capillary electrophoresis (HPCE) has been developed as a novel analytical technique since 1980. The separation of compounds is based on differences in migration velocity under a given electric field. Due to its high separation efficiency, high sensitivity, fast, small sample required, low consumption of chemicals and diverse application range, the HPCE has become one of an important technique and method in analysis of complex matrices.In this thesis, the development, current situation and applications of HPCE and capillary electrophoresis-mass spectrometry (HPCE-MS) were reviewed. The basic principle and characteristic of HPCE and HPCE-MS were introduced. The following aspects were investigated in the thesis:1. Micellar Electrokinetic capillary chromatography (MEKC) with indirect UV detection method was developed to analyze organic acids including malonic, formic, tartaric, malic, succinic, glutaric, acetic, lactic and glutamic acid in cane vinasse. The method is simple, rapid and does not require complicated sample preparation. Hence, the method is suitable for quick determination of organic acids in cane vinasse.2. A HPCE-MS method for analysis of tartaric, malonic, malic, lactic, succinic, glutaric and glutamic acid in cane vinasse was established. The method provides a better selectivity and identification compared to UV detection, which makes the analysis of organic acids in complex matrices more reliable.3. Two analytical methods for determination of sibutramine, indapamide, bumetanideand chlorothiazide in weigh-reducing tonic simultaneously were developed by HPCE and HPLC-MS. Two methods are simple, rapid, accurate and suitable for simultaneous determination of sibutramine, indapamide, bumetanide and chlorothiazide in weigh-reducing tonic.4. A HPCE-MS method for determination of apomorphine and sildenafil in medicines for Erectile Dysfunction has been developed. The method is high sensity, low operational cost and has been employed for determination of apomorphine and sildenafil in medicines for Erectile Dysfunction successfully.