Study And Application Of Druggery Analysis By Fluorimetric Method

The analysis of druggery is one of the most important components in the whole pharmacology category. The quantity of the druggery affects people's health and safety directly. Therefore the whole control of druggery plays an important role in the clinic and medical treatment. Spectrophotometry, chromatography and spectrophotometry-chromatography, as the most essential methods in the druggery measurements, have been widely used in the determination of pharmic products. In recent years, fluorimetry is developing in the study of analysis and druggery measurements, due to its highly sensitivity and good selectivity, simple procedure, less sampling and easy control apparatus. Obviously, fluorimetry is one of the methods in the quatitative determination of drugs in the modern instrumental analysis. Combination drug therapy means two drugs or more are adhibitted simultaneously or in sequence in clinic. The interaction between drugs will influence therapeutic effect and security of each drug. Therefore, it is important to establish method—simple, rapid and accurate in the druggery measurement and separation. Furthermore, much interference occurs in druggery analysis of biologic specimen among which albumins like urine albumin and serum albumin are the most severe. Therefore, it is the questions to be solved in the analysis of druggery to improve the accuracy, eliminate matrix interference and simplify the procedure. To improve the sensitivity and selectivity, some novel and simple methods for the assay of drugs have been established in my master paper. The first part of this paper reviews the application and prospect of derivative spectrophotometry in drug analysis in recent years. The synchronous fluorimetric method for the determination of carvedilol and ampicillin sodium without separation was discussed. Effects of pH, organic solvents and foreign ions on the determination of both drugs were examined. This method was rapid and simple and can be used for determining carvedilol and ampicillin sodium. The results obtained by this method agreed with those by the official methods. A sensitive and selective method for simultaneous determination of carvedilol and ampicillin sodium (AS) in the presence of human serum albumin (HSA) is described. The maximum emission wavelengths of carvedilol and AS are at 357 nm and 426 nm with the excitation at 254 nm, respectively. The determination of carvedilol and AS by normal fluorimetry was difficult in the presence of HSA because the emission spectra of carvedilol, ampicillin sodium and HSA were overlapped. Moreover, the synchronous fluorescence method cannot be used to determine the carvedilol or AS in the presence of HSA because of background of HSA. Fortunately, the first derivative fluorimetry can not only eliminate background interference of HSA, but also separate entirely derivative peaks of carvedilol and AS, This method is simple and can be used for determination of carvedilol and AS in human serum and urine sample with satisfactory results. The first derivative fluorimetric method for simultaneous determination of carvedilol and dopamine was described. The emission wavelengths of carvedilol and dopamine were at 354 nm and 314 nm with the excitation at 290 nm, respectively. The...