Photometric Research And Application In The Analysis Of The Amine Drugs

The analysis of druggery is one of the most important components in the pharmacology. The quantity of the druggery affects people's health and safety directly. Therefore the whole control of druggery plays an important role in the clinic and medical treatment. Spectrophotometry, chromatography and spectrophotometry-chromatography, as the most essential methods in the druggery measurements, have been widely used in the determination of pharmic products. In recent years, Spectrophotometry is developing in the study of organic analysis and life sciences. Obviously, UV-visible Spectrophotometry is one of the methods in the qualitative determination of drugs in the modern instrumental analysis. It is still the questions to be solved in the analysis of druggery to optimize the analytical conditions, improve the selectivity, reduce the detection limit and simplify the procedue.To improve the sensitivity and selectivity, some novel and simple methods for the assay of drugs have been established in my master paper.Spectrophotometric determination of procaine hydrochloride, ampicillin sodium and dapsone is disscussed. Procaine hydrochloride, ampicillin sodium and dapsone can react with 1,2-naphthoquinone-4-sulfonic acid to form salmon pink compound, respectively. Their maximum absorption wavelengtb.es were shifted from the range of ultraviolet to the visible light. Therefore procaine hydrochloride, ampicillin sodium and dapsone can be determined in the range of visible light. In this way, much potential interference may be avoided in the determination of these drugs in biological materials and hemanalysis. These methods are rapid, simple and wider linear range, and can be used for determining procaine hydrochloride, dapsone and ampicillin sodium in injectin solutions of these drugs. The results obtained by these methods agreed with those by the official methods.A sensitive and selective method for the determination of dopamine was studied.Dopamine was oxidized by mercury (II) nitrate and the oxidation product was condensed with ethylene diamine to form a quinoxaline derivative which was strongly fluorescent. The measurement was carried out at 447 nm with excitation at 393 nm. Effects of pH, oxidants, and foreign ions on the determination of dopamine were examined. A linear relationship was obtained between the relative fluorescence intensity and the concentration of dopamine in the range of 0.02 to 0.60 ug/ml, the relative standard deviation is 4.38%. The detection limit is 18 ng/ml, and the recovery is from 95.0 to 106.6%. Dopamine was separated from adrenaline and noradrenaline in urine by thin layer chromatography. This method has low detection limit and can be used for the determination of dopamine in injection and urine samples.The property of carvedilol in acid-alkaline medium was studied by spectrofluorimetry. A simple, rapid and high sensitive fluorimetric method for the determination of carvedilol in medicine is developed. The measurement of relative fluorescence intensity was carried out at 356nm with excitation at 254nm. A linear relationship was obtained between the relative fluorescence intensity and concentration of carvedilol in the range of 0.50 to 270 ng/ml. The detection limit of this method is 0.19 ng/ml. This method can be used for determination of carvedilol in tablet samples. The results obtained by this method agreed with those by the official method.