Combination Effects Of Prometryne And OT On Growth In Green Algae Chlorella Pyrenoidosa

The single and joint toxicities of prometryne and OT on growth of Chlorella pyrenoidosa were investigated. The chlorophyll content, soluble protein content as well as the density in the green algae cells were increased at the low concentration of prometryne and/or OT, which indicated that the algae growth was stimulated. Meanwhile, the activities of superoxide dismutase (SOD) and peroxidase (POD) were also increased, suggesting that reactive oxygen species was eliminated effectively. With increasing in concentration of prometryne and OT, the chlorophyll content, soluble protein content and the growth of algae were decreased significantly, while the activities of SOD and POD were increased initially, and then conceitedly fell. Additionally, the malonyldialdehyde (MDA) content increased rapidly. The results suggested that active oxygen was excessively and accumulated, and the lipid peroxidant was aggregated, resulting in damage of the algae cell membranes. 96h-EC50 of monotoxicity of prometryne was 0.164mg/l, and OT was 41.380mg/l The growth of algae was faster under both low concentrations than that of each agent alone, while the combinations of both high concentrations were more toxic than expected, which indicated that the both showed concentration addition, antagonistic and synergistic effects under the different concentration.