Preliminary Study On Technology System Of Rutin Determination And Extraction In Eucalyptus Youmanii

The Eucalyptus Youmanii which is rich in the rutin is introduced from Australia by the Forest-Science Academy of Sichuan Province as a rutin raw materials varieties in 1997.In this research the rutin content in leaf, bark and callus of Eucalyptus Youmanii are determined with Method with Direct Determining, Method with Aluminum Chloride and Method with Aluminum Nitrate and have compared it on the determining with HPLC.The crafts of rutin extraction from leaf of Eucalyptus Youmanii by Hot Water Extracting, Ethyl Alcohol Reflux Extracting , and Limewater Extracting are also discussed. These are favor of cultivating excellent variety; cultivating technical research and utilization of Eucalyptus Youmanii.There are some preliminary conclusions as following.A₂59/A₃64 of Methyl Alcohol extracting liquid after Aether degrease from leaves of different Eucalyptus Youmanii trees is higher than A₂59/A₃64 (1.152) of rutin reference substance. There are two A₂59/A₃64 Eucalyptus Youmanii trees, the one is 1.71, and another is 2.324. A₂59/A₃64 of leaf from the same tree with different rutin content is the same. Rutin content is widely different among Eucalyptus Youmanii, the greatest difference is up to 8. 5%. rutin content of leaves with A₂59/A₃64 (2.324) is obviously less than leaves with A₂59/A₃64 (1.71) So value of A₂59/A₃64 can be regard as one of the conditions of cultivating excellent variety of Eucalyptus Youmanii.259nm cannot be as detecting wavelength for Method with Direct Determining and HPLC.The rutin content determined at 364nm by Method with Direct Determining is also high. There are greater errors in determining Eucalyptus Youmanii rutin content by singleMethod with Aluminum Chloride or Method with Aluminum Nitrate, it is little error for sample with A259/A364 (2.324 ) by Method with Aluminum Chloride and for sample with A259/A364 (1.71) by Method With Aluminum Nitrate. But there are quite great errors for sample with A259/A364 (2.324) by Method With Aluminum Nitrate.The rutin content of Eucalyptus Youmanii leaves is from 4% to22%. But the rutin content of its bark and callus is extremely low, only under for 1%. The shorter the age of the leaf is, the higher the content of rutin is.. The leaves with complete growth should be plucked while harvest.When carry on the pretreatment to raw materials, with the rising of drying temperature, the rutin content is reduced; within 2-5 hours, drying time have a little effect on rutin content of the raw materials, best pretreatment condition is 60 °C, 4h.The crafts of extracting with hot water is time 30min—size 100 eyes —liquid rate 1:30, extracting twice, purifying with Ethyl Alcohol; The crafts of extracting with limewater is pH8—liquid rate 1:10—time lOmin, extracting twice , repeat crystal with limewater; The crafts of extracting with Ethyl Alcohol Reflux is 75% Ethyl Alcohol — temperature 75 —time 3h—NaHCO310%—liquid ration 1:20 and purify with hot water. But it has a large amount of pigment and phenol dissolving water in the raw rutin extracting with Ethyl Alcohol Reflux, rutin content is about 40%. Purifying with hot water is unsatisfactory. The method by limewater is better than others.