Interaction Between Phycocyanin And Se(Ⅳ),Au(Ⅲ),Ag(Ⅰ)

Nanoparticles complex modified by biomolecular is playing an increasingly important role.for example, Nanoparticles complex modified by noble metal nanopartical and protein are used in Immunocytochemical production,and so on. Nanoparticles complex modified by nanopartical and biomolecular can react selectively with virus, antigens and target cells.It is used widely in Immuno-labelling, recognition of biomolecules, DNA biosensor and gene therapy.In this paper, the nanoparticles complex of PC-Se(0), PC-Au(0) and PC-Ag(0) were prepared. The structure and formation mechanism of these complexes were also studied.Firstly, after C-phycocyanin(A620/A280=3.0, containing little allophycocyanin) was mixed with Se(Ⅳ), the spectrum of the mixture solution underwent changes and red nano-selenium was observed in the solution after 92h. The information from the spectra of phycocyanin suggested that phycocyanin could be one of the principal target molecules of Se(Ⅳ) and the biological effects of Se(Ⅳ) on spirulina, in part, could be caused accordingly by the interaction of phycocyanin and Se(Ⅳ). And a simple new method of preparing Nanoparticles complex were given.Secondly, PC-Au(0) is prepared from Au (Ⅲ) by in situ reduction of phycocyanin at room temperature without any reducing agent. PC-Au(0) is highly dispersed and stable,but the specific spectra of nano-Au(0) can not be observed on the reduction reaction.And thirdly,PC-Ag(0) is prepared from Ag (Ⅰ) by in situ reduction of phycocyanin at 4℃ without any reducing agent. According to the TEM images, the PC-Ag(0) is a nanostructured bioconjugate with novel core-shell, and the PC-Ag(0) is dispersed and stable and having 15~ 30nm nanoparticles of Ag(0).PC-Se(0), PC-Au(0) and PC-Ag(0) were prepared by in situ reduction of phycocyanin without any reducing agent and the mechanism of reaction were studied. Our results provide some useful inspiration in studing antioxidative protein and give a new method of preparing nano drugs.