Influence Of Core And Surface Chemisty Of Nanoparticle On Their Bioactivity

Due to the specificity in structure and size, nanoparticles have become the research hotspot in a large number of fields. The gold and silver nanoparticles are two of the most popular noble metal nanoparticles, and these two nanoparticles are liable to be controlled synthesized at specific size and morphology, and they are easy to be connected with functional groups by covalent bond so that their water solubility and biocompatibility can be improved. Therefore, these two nanoparticles have been studied more widely in the biomedicine application of nanoparticles. It is very important to study the factors of influencing nanoparticles’ biocompatibility in order to promote the biomedicine application of nanoparticles. There are a large number of researches which focus on the size, morphology and surface chemistry of nanoparticles; however, the influencing degree of core composition on the nanoparticles’biocompatibility has not been systematically studied. As a result, it is important to study the influence of nanoparticles’ core composition and surface chemistry on their physicochemistry and cell viability.By testing the physicochemical properties and cell viability of an array of spherical gold and silver nanoparticles with similar surface chemistry and size, this paper is aim to decide the more important influencing factor in determining the nanoparticles’ biocompatibility, core compositon or surface chemistry. And the detailed experimental program is as follows:First of all, design and synthesize nine kinds of organic molecule whose physicochemical properties are differ from each other and then connect them to the synthesized50nm spherical gold and silver nanoparticles. Make sure the surface ligand density of gold and silver nanoparticles with the same ligand is similar, so that we can ensure their surface chemistry are similar.Then we test the nanoparticles’ physicochemical properties (DLS and zeta potential) and cell viability (HEK293and A549). The result showed that the physicochemical properties of the gold and silver nanoparticles with similar surface chemistry are very different in water, but when in cell culture media, they are similar. The gold and silver nanoparticles with similar surface chemistry showed some differences in cytotoxicity, which indicated that nanoparticles’ cell viability is influenced both by their core composition and surface chemistry, while the surface chemistry is much more important.In summary, both core composition and surface chemistry of functionalized nanoaprticles will influence their physicochemical properties and cell viability. However, the core composition and surface chemistry of nanoparticles modified with different ligands show different influence degree, which is connected with the ligands’ physicochemical properties. The surface chemistry of functionalized gold and silver nanoparticles used in this paper influence much more than the core composition on their physicochemical property and cell viability. These findings can provide reference and direction for the functional design of nanoparticles to be applied in biomedicine.