Eastablish And Application Of The Caries Protection Assessment Program

Dental caries is a kind of wide spread human diseases, and has been ranked in the three diseases emphasized to be prevented and treated. Prevention and remedy of dental caries, as a public health problem, have been received more and more attention. To solve it radically, prevention must be grounded on. In recent years, many functional foods to prevent it have springed up and developed rapidly. Up to now, the manufacture scale and sale volume of anticaries food are enlarged day after day, while the function assessment and normalized administration lags behind. Unitary standards to assess such foods all-sidedly and scientifically still need to complete. In the 24 items of health foods assessments issured by health ministry, the ones evaluating the function of caries protecting food were not involved in. Based on the the authority literatures of FDA and ADA and "technical standards for testing and assessment of health food"(2003), a series of function assessment procedures and testing methods of caries protection foods were established, and a kind of caries protecting food, developed from a specific antibody of fowl yolk was used to verify the objectivity, all-sidedness and operability of our methods. Methods: The security of the sample was evaluated according to "technical standards for testing and assessment of health food"(2003) GB 15193.1-15193.19—94. Streptococcus mutans (S. mutans) were cultured with the sample of olitin tooth protecting spray. Then S. mutans were viewed through spectrophotometer and the percentages of the adherent S. mutans were calculated in vitro. 40 wistar rats were divided into 4 groups: group A as control group; group B, C, D were dealed with our sample in low, middle, high dose respectively. They were all fed with caries-inducing diet 2000*. The sums of the caries scores were assessed by the procedure of Keyes'.Results: The LD50 of the sample was more than 20ml/ kg. BW, and there were no significant difference between the numbers of micronuclens of born marrow cell and sperm shape abnormality of test groups and control. There were no distinctive effects of the sample on blood test, body weight and biochemical index. All these indicated that the sample has no toxicity to the body.It was showed that the growth of S. mutans was not inhibited, but the long chain was occurred. The adherence of S. mutans cells on the glass decreased, the pH value increased to above 6.0. There was a significant lower mean of caries score of test group than thoseof the control group (p<0.05).Conclusion: It was demonstrated that IgY preparation was nontoxic and may be added infood. The sample could prevented efficiently the occurrence and progress of caries in rats,the mechnism of the pretection lied in the enhancement aggregating, inhibition adhesionand decreasing sour products of S. muntans.It was also proved that the function evaluation procedure and testing methods could givea scientific, all-sideness, and operative assessment to foods function, so they providedtheoretic references and application experiences to assess caries protecting foods andenhanced supervision and administration of anticaries food.