The Study On The Synthesis And Fluorescent Properties Of New Time-resolved Fluoroimmunoassay Chelator

TRFIA is a new immunoassay technology established in 1970s.Using the chelator of rare-earth ion labeling Ag or Ab,the high sensitivity of rare-earth ion detection can be obtained with time-resolved fluorometry because of the fluorescent character of chelator:exceptional fluorescence life and large Stokes'shift.When measuring fluorescence,using befitting time and measuring wave,the disturbance of background can be eliminated effectively. There exists two directions:DELFIA and DSLFIA in the field of TRFIA research.DELFIA is named by using enhanced-solution when measuring.High sensitivity can be obtained with DELFIA,but the operation is inconvenient and easily contaminated by the rare-earth ion existed in environment.DSLFIA is widely concerned because of operating conveniently and the appling foreground in DNA hybridization assays and immunoassay fluorescence image.The chelator used in DSLFIA should not only have the bi-function properties:conjuncted with protein and chelated with rare-earth ion,but also transfer the energy to rare-earth ion.An ideal chelator is the key technology of DSLFIA. BHHCT and BTBCT are the kind of compounds comprising o-terphenyl, β-diketonate and –SO2Cl,could be easily conjuncted with proein by –SO2Cl and chelated by β-diketonate,and transfer the energy to rare-earth ion high efficiently.The paper related about the internal and exterior progress of...