| With the coastal population and animal husbandry increasing, fecal contamination has become a serious environmental problem that affected many coastal and inland waters worldwide. It will degrade water quality and influence seawater breed aquatics, as well as recreational activities, such as pathogens associated with fecal pollution can lead to human disease and economic losses in industries that depend on coastal waters. The problem persists, partly due to an inability to reliably identify non-point sources, which are dispersed and sporadic. Identification of the source of the fecal contamination is the key of the solving fecal contamination of seawater.In order to solve the problem of fecal bacteria in contaminated waters, in this paper, microbial source tracking (MST) is presented. Compared with three methods of MST, Molecular (genotype) are more reliable, which referred to as "DNA fingerprinting", are based on the unique genetic makeup of different fecal bacterias. In this paper, E.coli is chosed as indicator of fecal contamination source tracking, and fecal samples will be collected, including human, cattle, swine, and chicken, from Jinzhou bay. In this research, the genomic DNA is extracted from the E.coli of samples, used 16S-23S rRNA intergenic spacer region (ISR) -PCR, combined with DNA-polyacrylamide gel electrophoresis (PAGE) as tools of analysis E. coli isolates "DNA fingerprinting", try to identify different fecal sources' E. coli fingerprinting profile.In the experiment the proper conditions of PCR and PAGE are successfully groped Furthermore, according to PAGE results, the special fingerprinting strip of different sources can be found. Then sources of fecal contamination can be basically distinguished. In conclusion, This paper gets hold of a set of methods, which will be used by regulatory agencies in the future, to identify the fecal source, control the contamination, and predict health risk.
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