Study On Synthesis And Characters Of Alkaloids Molecular Imprinting Polymers

Molecular imprinting technique (MIT) is a borderline subject developed in recent several decades. It developed from combining organic chemistry, high polymer chemistry and biochemistry. MIT is a new technique of simulating mechanism of receptor-antibody. MIT has been applied to many fields which include the use of it as tailor-made separation materials, antibody and receptor binding site mimics in recognition and assay systems, solid phase extraction and recognition elements in biosenors. The fundamental principle of molecular imprinting technique, synthesis and characteristics of molecularly imprinted polymers and application fields developed tendency of molecular imprinting technique are briefly discussed. The simple way of separation from herb medicine was abserved, and the contents of available composition of alkaloids were analysed validly. In this study, Molecularly imprinted polymer microspheres (MIPMs) for high performance liquid chromatography (HPLC) were synthesized by aqueous micro-suspension polymerization with polyvinyl alcohol 400±50 as dispersive reagent, using caffeine as imprinting molecule, MAA as function monomer and EDMA as cross-linker. The major factors that influence the sizes and size distribution of the MIPMs were discussed, and the influences of pH of mobile phase on the capacity factor, separation factor and selection factor of caffeine were investigated in detail. The results showed that the caffeine imprinted MIPMs had a considerable property of molecule selecting to caffeine with the separate factor up to 1.6 when theophylline was used as competitive molecule, and the caffeine of tea was separated and enriched. The molecularly imprinted polymers were synthesized by aqueous polymerization and their selective binding characteristics were shown,using berberine hydrochloride as imprinting molecule, ACM as function monomer. The results showed that the MIPs had high affinity and specificity to berberine hydrochloride when the methyl-berberine hydrochloride was used as a competitive substrate. The dissociation for substrates indicated to be K=1.0×10-4mol/l. BH of berberine pills was recovered, and the rate of recycle is 85%.