A review has been accomplished for the advances in natural products' separation by supercritical fluid chromatography (SFC) in recent years.The research work and separation methods of arachidonic acid ethyl ester(AA-EE) were also reviewed. By comparison of advantage and disadvantage of the separation methods, we selected SFC to separate AA-EE from PUFAs oils.AA-EE and linoleic acid-EE could be successfully separated by SFC at the range of 10 MPa |