| The analysis of nucleic acids plays a very important role in life science. It is widely used in molecular biology, biochemistry and medical diagnostics. Nucleic acid probes can selectively bind to its target molecules, which include not only nucleic acids but also protein and drugs.In the thesis, a chemiluminescence method to detect nucleic acid labeled with Cy5 was developed, which provided a sensitive method for the determination of its target molecular. Meanwhile, we also made use of gold nanoparticle-labeled DNA aptamer to detect human a-thrombin, and a new method based on nucleic acid probe and Resonance Light Scattering (RLS) was established. This thesis consist of two parts:Part one: Study on chemiluminescence detection of Cy5-labeled nucleic acid probe Cy5 is a nice fluorescence dye, and it has been widely used in labeling nucleic acid and antibody. However, its fluorescence signal usually interfered by the scattering signal of the incidence light, which result from small Stokes shift of Cy5. We developed a new method based on TCPO-H2O2 chemiluminescence to determine this nucleic acid probe, and the optimum analysis conditions have been investigated. There was good linear relationship between the CL intensity and Cy5-labeled nucleic acid in the range of 8.4 ×10-92.8× 10-6mol/L, and the detection limit was 2.8×109mol/L. A sensitive and convenient new analysis method for the determination of Cy5-labeled nucleic acid was accordingly established.Part two: Study on the Resonance Light Scattering analysis to determine thrombin by using gold nanoparticle-labeled DNA aptamer as probe.Aptamers are single-stranded oligonucleotieds that can bind with high affinity to their respective target molecules such as nucleotides, drugs, and proteins. We have chosen two DNA aptamers of human a-thrombin, and labeled it with gold nanopaticles. When the DNA probes mixed with human a-thrombin , the gold nanoparticles can self-assemble to form a...
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