Study Of Analytical Methods For The Drugs (Tiopronin Etc.) In Biological Samples

This article mainly established analytical methods for determination drugs or their metabolites in plasma by high performance liquid chromatography coupled with electrospray ionization mass spectrometry or ultraviolet, and hence to supply reliable data for pharmacokinetics study. Moreover, molecular imprinted polymers by synthesizing were loaded to the solid phase extract cartridge to provide a high selective method for the pretreatment of biological samples.This study has mainly finished the following work:1. An analytical method was developed for determination of tiopronin in human plasma by high performance liquid chromatography/electrospray-mass spectrometry. Tiopronin was free from human plasma after using ascorbic to dissociate disulfides. Tris reagent was chosen to change the retention behavior of tiopronin and improve the separation. High sensitive ESI-MS detector was selected to determine tiopronin in the human plasma, which was successfully applied to the pharmacokinetics study.2. An analytical method was developed for determination of flnasteride in human plasma by high performance liquid chromatography/electrospray-mass spectrometry. Recovery was improved by adding proper amount of ammonia to samples before liquid-liquid extraction. High sensitive ESI-MS detector was selected to determine finasteride in the human plasma, which was successfullyapplied to the pharmacokinetics study.3. An analytical method was developed for the bioequivalent study of valaciclovir through high performance liquid cliromatography-ultraviolet (HPLC-UV). Perchloric acid was chosen as the protein precipitator in plasma samples. Acyclovir (the metabolite of valaciclovir) was selected as the objective, which was successfully applied to the pharmacokinetics study.4. An analytical method was developed for the bioequivalent study of aspirin tlirough high performance liquid cliromatography-ultraviolet (HPLC-UV). Recovery was enhanced by adding proper amount of sodium hydroxide in Perchloric acid. Phosphate buffer was chosen as the mobile phase to improve the shape of peak for salicylic acid. Salicylic acid (the metabolite of aspirin) was selected as the objective, which was successfully applied to the pharmacokinetics study.5. Molecular imprinted polymers of diethylstilbestrol were synthesized as the packing materials in solid phase extraction cartridge, which were proved of good selective concentration effect for diethylstilbestrol and were successfully applied in pretreatment of biological samples.