Research Of An Adsorbent For Hemopurification

The studies of an Adsorbent for Hemopurification were carried out in this dissertation, which consists of three parts.The first part is a review concerning the requirement, the classification, the usefulness and the research evolution of the materials for Hemopurification. Then some researchreports were given in the second part. The third part is a conclusion.1. Preparation and in Vitro Studies of Cross-linked Agar BeadsEntrapped Attapulgite ClayA new method for preparation of Cross-linked agar beads entrapped Attapulgite clay (CAA) was reported. Attapulgite clay was coated with agar and shaped in organic solvent, as well as cross-linked by epichlorohydrin. The products withstood autoclaving at 121°C for 30 minutes and had a good adsorption ability for model compounds and medicines. The results of preliminary investigation indicate that CAA is relatively hemocompatible.2. The Adsorption Capacities of Crosslinked Agar Beads EntrappedAttapulgite Clay (CAA)The adsorption capacities of Crosslinked Agar bead entrapped Attapulgite clay (CAA) for certain poisoning drugs, such as chlorpromazine, promethazine, diazepam, quinidine, phenobarbital are reported. The results revealed that CAA possessed different absorptivity for the different drugs, and possessed strong adsorption capacities for some cations, such as phenothiazine derivatives. It is a selective adsorbent with good adsorption capacities.3. Determination of a Trace Level of Methylene Blue in Human Plasmaby Combination of Solid-phase Extraction and SpectrophotometryA spectrophotometry method was developed for the determination of a trace level of methylene blue (MB) in human plasma. Plasma proteins precipitated by acetone. MB was extracted and cleaned up on silica gel G solid-phase extraction cartridges. MB concentration was determined by spectrophotometry at 653nm. The average recovery of MB from human plasma at 20-100 ng-mL'1 were 96%~102 % (RSDs, 2%~9 %). This method could separate and concentrate MB from human plasma efficiently, and the extraction rate was high and stable. The mass of plasma can be addition or reduction with the different concentration of MB in plasma. This method is simple, and has good accuracy and reproducibility. It is suitable for the determination of MB at ng-mL'1 level in human plasma.4. Determination Phenothiazine in Blood by A Solid-phase Extractionwith Alumina Dry Column ChromatographyTo establish a method for extracting and determining phenothiazine drugs in blood, isolated phenothiazine drugs from blood by solid-phase extraction with alumina dry column chromatography. The phenothaizine drugs in extractives was determined by UV 2nd derivative spectrogram. The extraction rate was >80% for chlorpromazine (pH=8), and >90% for promethazine (pH=12) respectively. The recoveries of chlorpromazine from blood were 93.0~99.9 % (RSDsl~4%, n=4). The method is simple, convenient and low cost. It has a good repeatability and higher extraction rate, especially suitable for dealing with the batch samples. The method is reliable for determination of phenothiazine drugs in blood with the level of mg-L"1.5. Preparation and Studies of a Adsorbent Double Cross-linked AgarBeads Entrapped Attapulgite ClayA method for preparation of double cross-linked agar beads entrapped attapulgite clay for hemopurification is reported. Attapulgite clay was coated with agar and cross-linked by epichlorohydrin. After drying-out the cross-linked agar beads entrapped attapulgite clay (CAA), cross-linked it by 10% toluene 2,4,-diisocyanate in acetone for 3.5h , 35°C. The products was withstood autoclaving at 121 °C for 30 min. The performance tests manifested that the adsorption of the double cross-linked agar beads entrapped attapulgite clay (DCAA) was about 4 times of the adsorption of CAA to methylene blue. The intensity of DCAA was raised 6 times, and the appearance was more uniform and smooth. Fully contacted with blood lh, the lost of leucocyte was <1%, of erythrocyte was <5%, and of blood platelet was <8%. Fully contacted with blood 2h, the lost of leucocyte was <2%, of erythrocyte was < 10%, and of blood platelet was <20%.