Influence Of Accompanying Anions On Activities Of Plasma Membrane Ca～(2+)-ATPase From Lead Stressed Vicia Faba Seedlings Root

A laboratory experiment was conducted to evaluate the influence ofaccompanying anions on toxicity of lead in Vicia faba seedlings root using test theactivities of plasma membrane Ca²⁺-ATPase (PM—Ca²⁺-ATPase). Lead withdifferent accompanying anions was carried out, they were lead chloride (PbC12),lead acetate (Pb(OAc)₂) and lead nitrate (Pb(NO₃)₂) respectively. The Pbconcentrations were 0, 1×10^-7, 1×10^-6, 1×10^-5, 1×10^-4, 1×10^-3 mol/L in solutionswhich used to expose Vicia faba seedlings root to lead respectively.And theexposedtimewas12,24,36hoursrespectively.A standard protein curve and a standard phosphorus curve suitable for thisexperiment were worked out. Effect of Ca²⁺, ATP, Temperature, and pH on theactivityofPM—Ca²⁺-ATPasefromViciafabaseedlingsrootweredetermined.Low-temperature high-speed centrifuge Beckman J2-21 (rotor: JA-21) andaqueous two-phase extraction (ATPE) were used to obtain highly purified PMvesicles. The PM—localized Ca²⁺-ATPase activity was tested. And the influence ofaccompanying anions on activities of PM—Ca²⁺-ATPase from lead stressed Viciafabaseedlings rootwererespectivelytested.The result of activity of PM—Ca²⁺-ATPase from Vicia faba seedlings roottesting demonstrated that, the activity of PM—Ca²⁺-ATPase reached the maximumwhileCa²⁺concentrationwas3mmol/LandATPconcentrationwas3mmol/L...