Study On Compound Huzhang Fengfang Spray

Objective: To study on the pharmacodynamics of Compound Huzhang Fengfang Spray (CHFS), which was a preparation of modern compound Traditional Chinese Medicine for curing second degree and small area third degree burn.Methods: The optimum extracting procedures of Polygonum cuspidatum and Nidus vespae were selected by orthogonal test design. The guide component of Polygonum cuspidatum or Nidus vespae was determined by HPLC or UV method. Screening the dosage of the single medical material and the compound preparation, the optimum prescription was obtained. Designing the per-cutem osmosis test, the osmosis process of CHFS was understood. Using Burn Spray (BS) as the contrast, experiments of Dimethyl Benzene-induced acute mouse ear oedema and egg-induced paw oedema in rats for anti-inflammatory, hot plate test and writhing test in mice for analgesia, the plate method and the tube double dilution test for anti-bacterial, deep second degree and small area third degree burn wound healing in rats and hydroxyproline (Hyp) determination were designed. By those CHFS was studied on its main pharmacodynamics.Results:â‘ The optimum extracting procedure of Polygonum cuspidatum was A₃B₂C₃D₁, i.e. extracting with 20 times ethanol (95%) 3 times for 3 hours.â‘¡For Nidus vespae the optimum extracting procedure was A₂B₂C₃D₂, that is, extracting 3 times with 20 times water for 4 hours, and the extract was concentrated and precipitated by adding ethanol to concentration of 60%.â‘¢The optimum prescription of CHFS was Polygonum cuspidatum 0.10 g·ml^-1, Nidus vespae 0.10 g·ml^-1 and Borneolum Syntheticum 0.5%.â‘£The skin accumulation was 3.99 times...