Degradability And Theoretic Study On Diesel Oil In In-Situ Bioremediation Of Contaminated Soil

Petroleum is one of the most important energies and also a backbone of the national economy. But today petroleum pollution, especially oil-polluted soil due to the leakiness during the process of producing, transporting, storing, and using, is becoming one of the most serious problems. More and more countries pay attention to this problem, and various efforts have been taken to reduce its influence. Bioremediation techniques for oil contaminated soil have been the hotspot in environment protection field. And in-situ bioremediation is conceived the most useful technique with the advantages of economic, no second-pollutant, high efficiency, and little influence to soil itself.In this paper, based on the in-situ bioremediation technology, the two phase flow of air-liquid in the soil and the in-situ bioremediation for diesel oil in the soil were researched in the visual experiments. Besides, a theoretic model on the biodegradation for the polluted soil was set up to investigate the influences of two phases flow in soil on bioremediation. . The main concludes are listed as follow:1 Based on the in-situ bioremediation technology for the soil empoisoned by diesel oil, the mixed animalcule bacteria with strong ability of degrading diesel oil were obtained by screened from oil-containing soil and then domesticated by using diesel oil as sole carbon source in the laboratory. The influences of temperature, pH value, and the substrate concentration on the biodegradation efficiency were researched experimentally. Besides, the two important kinetics parameters of Michaelis-Menten equation were also calculated out with the Michaelis-menten constant.2 The characteristic parameters of the studied soil, such as the soil permeability, the porosity, particle diameter and so on, were measured.3 The liquid saturation in the soil is increases with the depth of the soil increases under the condition of liquid penetrating through the soil from the top and air penetrating through the bottom. The contaminations of diesel oil in the soil are biodegraded by the microorganism, and the diesel oil will not arrive below the depth 40cm of the soil under the condition of the Cw being 15%~20%. The biodegradation efficiency is 60% after 26 days. For the same liquid thickness in the infiltration gallery, the rudimental content of the diesel oil in the soil with the...