Screening Of Epoxide Hydrolase Producing Strain And Study On Its Fermentation And Conversion Technics

Epoxide hydrolase stereoselectively hyrolyzes epoxy substrates to pure optical epoxides and vicinal diols. The producs are important synthesization intermediates in pharmaceutical, pesticide, spicery and fine chemical industries. Producing chiral epoxides and vicinal diols by means of enzymes is a very attractive method, which has high stereoselectivity, and is good for environment. Searching new enzyme source from microbe is the focus in this field. The microbial epoxide hydrolase can preferentially translate (R)-epoxy substrate to (R)-vicinal diol, actualizing the highly efficient separation of racemic epoxides.Epoxide hydrolase producing strain J-14 was screened from soils of different areas and lab strains. Its growth curve, heredity stability were determined. It was identified as Bacillus spp.Based on single factor experiments, the optimal compositions of...