| Because marine micoorganisms are rich in resource, they are important sources of antibiotics. Their massive metabolites with unique structures and excellent bioactivities have become the resource treasury of natural drugs, thereby they have turned to be the hotspot of new drug research in the world.Marine sulfate-reducing bacteria(SRB) comprise a special group of marine microoganisms. They are strict anaerobic bacteria and can produce energy by a metabolic pathway without O2 as an electronic acceptor. Compared with the aerobic bacteria, their secondary metabolites should have many differences because of their different enzyme systems. Thus they show great potential in new antibiotics finding.16 sea water samples and 9 sea sediments were collected from near-shore of Dalian. From them 125 SRB strains were isolated and 70 pure SRB strains were tested for the antibacterial and antifungal bioactivities. It showed that 10 SRB strains had prominent antibiotic bioactivities and accounted for 14% active rate. It is obviously that marine SRB have the potential of medicine exploitation.Isolated from sea water of Hei Shi-jiao sea area, SRB 18 exhibited strong inhibitive activity against Bacillus subtilis. By studying the morphology, cultural characteristics, physiological and biochemical properties and phylogenetic tree, SRB18 was identified to be Desulfovibrio desulfuricans.Through culturing, 30L fluid of SRB18 was obtained. The cultured fluid was concentrated to 1000ml by reduced pressure distillation. The active metabolites were isolated and purified primarily using methods of solvent extraction, TLC and HPLC, the result showed the purification product had prominent bioactivity inhibiting Bacillus subtilis.
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