Hydrolysis Of Rice Residue Protein And Synthesis Of Complex Amino Acid With Calcium

Rice residue protein is the main wastes generated largely in monosodium glutamate industry and it is good and cheap source of protein and amino acid. It is usually used as feed additive because of its cheapness. But it will incarnate higher value if being used in human food industry. We know that calcium deficiency is a big problem for many Chinese people and amino acid chelated mineral is the trends of nutrition complement, so we researched using rice residue protein to prepare complex amino acid calcium.The effects of alkali protease, acid protease and papain were compared. The result showed that the percent hydrolysis of alkali protease was much higher than the others. Premium working conditions for the enzymolysis were determined by means of orthogonal experiments. The best conditions of enzymolysis were as follows: pH was 10.5, time was 7 hours, enzyme dosage was 1.50% and temperature was 60℃. In such conditions the content of complex amino acid in the hydrolysate was 1.39 gram per 100mL and the transformation ratio of amino acid was 23.76%. The best conditions of acid hydrolysis were as follows: the time was 11 hours, the ratio of raw material mass to acid volume was 1:7, and the concentration of hydrochloric acid was 6 mol·L-1. An ordinal rank of influence was time, the ratio of raw material mass to acid volume and the concentration of hydrochloric acid. The percent hydrolysis was 70.8%. Obviously, acid hydrolysis was superior because of its high utilization ratio of rice residue protein. The color of acid hydrolysis liquid was brown and it...