| Rice residue is by-product of rice syrup industry.The rice residue being at present sold to the feed mills and farms,that makes a huge waste for its over 60% high quality rice protein.The chelated peptide ferrous was obtained from a reaction by inorganic ferri chelated with rice protein peptide through enzymolysising rice residue.The nutritional value and application were elevated in our researches.The purity of the rice protein was determined by measuring the contents of cellulose,starch,fat on rice residue,and the purity and contents of the peptide could be improved by pre-treatments.The pre-treatments was first by extracting fat from raw materials by soaked residue for 40 minutes in a ratio of 1 to 1 of rice residue to ligarine solvent.Dried defated residue was then washed twice by a 5 times volme of water at 50℃for 30 minutes.The a-amylase and cellulase were both used by 0.5% for 30 minutes to hydrosise starch and cellulose in pH6.0 and pH5.0 at 50℃, respectively.The protein content of pre-treated residue could reach to 81.05%.1‰of parenzyme was chosen to hydrosis pre-treated rice residue by comparing 4 different proteinases according to the single factor and Design-Expert software.A regression mathematical model was established with the parameters of temperature X1, liquid-solid proportion X2,pH X3,enzymolysis duration X4,degree of hydrolysis(Y). A regression mathematical model to multi-dimensional return and to the degree of hydrolysis(Y) was established as follows: Y=8.93+0.27X1+0.056 X2+0.039 X3+0.28 X4-0.63 X12-0.13 X22-1.25 X32-0.19 X42-0.0025 X1 X2+0.078 X1 X3+0.015X1 X4+0.035 X2 X3+ 0.005 X2 X4+0.02 X3 X4The optimal parameters of hydrosis rice residue were determined through the model analysis.The degree of hydrolysis was reached to 8.97%by using treatment of temperature at 50.03,the liquid-solid by 1:10,pH in 8.04,and hydrosis duration for 2.96h.The mathematical model has significant regression(P＜0.0001),no significant loose imitate with the R2=0.9706,Radj2=0.9413 and being showed a significant effects in practical and linear relationship between independent and response variables.The allotri-Vc-Na method was eventually chosen to improve the properities of denatured proteins of rice residue by comparing acid,enzymic,allotri-Vc-Na methods. The optimal parameters of the allotri-Vc-Na method were obtained for 60min at 40℃of pH 5.0 by adding a 0.15g of allotri-Vc-Na.The properities of denatured proteins has a oil absorption for 90min,40℃,pH 5.0,adding quantity 0.10g;emulsibility for 90min with a optimal treated at 40℃in pH 7.0 with 0.15g allotri-Vc-Na,and a stability of emulsification for 60min treated at 60℃in pH9.0 with 0.15g allotri-Vc-Na.Both functional and physiological activities of proteolysised denatured ricr protein including solubility,stickiness and particle size distribution had a significant improvemen compared to original denatured protein.The produced protein showed a good stability in thermal and cold treatments.The short chain peptide of rice protein showed that the product had a elimination ability to three kinds of free radicals, including DPPH·,·O2- and·OH.The clearancing rates were 62.36%,82.07%,35.41% for three free radicals at concentration of peptide at 10mg/mL,respectively.The rice protein peptide was obtained pilot plant scale production under the optimal enzymolysis conditions.The protein content of it could be achieve to 91.05%, and a yield of peptide was 50.3%.The end product of rice protein peptide had a main distrubution of molecular weight under 3000Da,the short chain of peptide content being 77.87%with a essential amino acid of 39.1%.The optimal conditions for ferrous chelator production were obtained through the single factor and the orthogonal experiment.The optimal parameters are as following: the weight ratio of polypeptide and ferrous sulfate at 2:1,pH at 6.5,temperature of chelation reaction at 35℃for 25 min.The pilot plant scale production was investigated under the optimal laboratory condition.There was no significant difference among the products of pilot,lab and inported products of ferrous chelator.
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