Study Of Interface Adsorption Behavior And Its Application By Total Internal Reflection Synchronous Fluorescence Spectroscopy

Porphyrin plays important role on biological chemistry process, since they interact with protein, adsorb to or penetrate membrane. So it is significant to study their character of interfacial behavior. Total Internal Reflection Fluorescence (TIRF) Spectroscopy is an effective method to investigate interface on molecular scale. And synchronous fluorescence spectroscopy can improve selectivity and simplify the spectrum. Combining those two techniques above can successfully decrease the scattering light from interface and simplify spectrum. This dissertation is concerned on the investigation of meso-tetrakis (4-sulphonatophenyl) porphyrin (TPPS) interaction with bovine serum albumin (BSA) and different surfactants, and their adsorption behavior on interface by Total Internal Reflection Synchronous Fluorescence (TIRSF). The dissertation is composed of following parts:In the first chapter, the principle and advantage of total internal reflected fluorescence was introduced, and other techniques for interfacial study were reviewed. The TIRF applications on water-solid, liquid-liquid interface research were described in detail. Research about interaction between protein and porphyrins was introduced. The plan of dissertation was put forward at the last part of this chapter.In the second chapter, a new method to determine protein concentrations was established, based on the synchronous fluorescence from water-solid interface...