Study Of Adsorption Of Proteins And Antibacterial At Liquid-liquid Interfaces Using The Total Internal Reflected Resonance Light Scattering And Their Analytical Applications

When a light beam passes through a liquid/liquid interfaces and is totally reflected from the interfaces between the optically rarer and denser phase, it develops an evanescent field on the side of an optically rarer phase at the interface. The evanescent wave rapidly decays with increasing distance from the interface, typically within a distance of one wavelength. The total internal reflected resonance light scattering (TIR-RLS) is constructed on the basis of measurement of resonance light scattering (RLS) at H2O/CCl4 interfaces with the total internal reflection of light. In this thesis, the interfacial reaction between the complex of metal ion and the synergistic ligands and the electrostatic coadsorption are investigated by the total internal reflected resonance light scattering. With this, we present assay methods of protein and antibacterial with sensitive and selective, and studied the mechanism, the affecting factors.In the pH range 3.29-3.78, the interaction of Evans blue(EB) with proteins in the presence of Cetyltrimethylammonium bromide (CTMAB) results in strong enhanced TIR-RLS signals at H2O/CC14 interfaces. The enhanced TIR-RLS intensity at 360-370nm was found to be proportional to the concentration of protein in the range 0.07-1.2u?ml. The limit of detection (3cr) is 6.30ng/ml. The content of protein in human urine samples can be directly determined with high sensitivity.A direct quantification of human serum albumin (HSA) in blood serum samples without separation is proposed based on the measurements of total internal reflected resonance light scattering (TIR-RLS) occurred at a water/tetrachloromethane (FkO/CCU) interfaces. In the pH range of 6.37-6.59, the coadsorption of the binary complex of HSA-Th(IV) with Sodium dodecylbenzene sulfonate (SDBS) occurs at the H2O/CCl4 interface, forming an amphiphilic layer and displaying in greatly enhanced TIR-RLS signals with the maximum peak located at 340-370 nm. The enhanced TIR-RLS intensity is in proportion to the HSA concentration in the range 0.15-1.0g/ml. The limit of detection is 14.4 ng/ml. The content of HSA in bloodserum samples were determined with the recovery of 97.1-102.3% and RSD of 0.6-2.9%, which are identical to those obtained according to the spectrofluorimetric method using chrome azurol S.A simple assay of penicillins such as ampicillin (AmP), benzyl penicillin (BP), Oxacillin (OA) and amoxycillin (AmO) was developed based on the measurements of enhanced TIR-RLS signals of penicillins in the presence of CTMAB at H2O/CCl4 interfaces. In neutral solution, the coadsorption of penicillins with CTMAB occurred at H2O/CC14 interfaces, displaying in greatly enhanced TIR-RLS with the maximum peak located at 368-372nm. The enhanced TIR-RLS intensity is in proportion to the penicillins concentration in the range 0.15-2.2+10-6mol/l, and the limits of detection for AmP, BP, OA and AmO were found to lie in the nanogram range. The pharmacokinetics of oral ampicillin such as half time (t1/2) and excremental quantum among 8 hours were obtained according to the assay penicillins in human urine using the present method.In the pH range of 2.6-5.7, the coadsorption of the berberine with the anionic surfactants such as sodium dodecyl benzene sulfonate (SDBS), sodium dodecylsulfonate (SDS) and sodium lauryl sulfate (SLS) occurs at the H2O/CCl4 interface, resulting in greatly enhanced TIRF signal characterized by the emission at 526 nm when excited with a 351 nm light beam. The enhanced TIRF intensity is in proportion to the berberine concentration in the range 0.2-10.0+10-7mol/l. The limit of detection is 1.7+10-9 mol/1 (3a). In aqueous solution at pH 5.33, the coadsorptin of penicillins such as ampicillin (AmP), benzyl penicillin (BP), Oxacillin(OA) and amoxycillin (AmO) with berberine chloride (BBC) occurred at H2O/CCl4 interfaces, displaying displaying in greatly enhanced TIR-RLS signals with the maximum peak located at 370 nm. The enhanced intensity of TIR-RLS is directly proportional to the concentration of in the range of 0.98-10.0+10-...