The Interaction Mechanism Of Phenanthroline Complexes Of Nickel (Ⅱ), Copper (Ⅱ) And Cobalt (Ⅱ) With DNA And The Investigation Of DNA Electrochemical Biosensor

In this paper, with 1,10-phenanthroline and 1,10-phenanthroline ramification which used as ligands, nickel (â…¡), copper (â…¡), cobalt (â…¡) metal complexes have been synthesized successfully. The structures of these three novel complexes have been determined by means of crystalline structure determination, IR and EA, respectively. Reaction conditions, interaction modes, binding ratios and binding constants between metal complexes and DNA have been determined by means of electrochemical and spectroscopic methods. With [Cu(phen)₂IP]·2ClO₄·3H₂O (phen = 1,10-phenanthroline, IP = imidazo [f] 1,10-phenanthroline) and [Co(phen)₂IP]-2ClO₄·3H₂O as hybridization indicators, the DNA electrochemical sensors via covalently immobilized DNA modified glassy carbon electrod have been prepared, respectively.(1) With 1,10-phenanthroline as ligand, nickel (â…¡) metal complex has been synthesized, and its structure has been determined by means of crystalline structure determination, IR and EA. The interaction mechanism between metal complex and DNA has been studied by means of electrochemical and spectroscopic methods. The results showed that the compound formula is [Ni(C₁₂H₈N₂)(C₈H₄O₄)(H₂O)₃]·H₂O. The interaction mode between metal complex and DNA is intercalation and the binding ratio is 1:1 and the binding constant is 8.28 × 10² L·mol^-1.(2) With 1,10-phenanthroline and 1,10-phenanthroline ramification as ligands, copper (â…¡), cobalt (â…¡) metal complexes have been synthesized, and their structures...