Study On The Bioactive Substances In Fujian Yellow Rice Wine

In this study, the nutritional components in Fujian Yellow Rice Wine were analyzed. Some bioactive substances, in particularly the components with anti-ACE activity and antioxidative activity were isolated, purified and identified. The physiological fuctional mechanism of Fujian Yellow Rice Wine was discussed.The content of protein, total acid, amino acid-N and total reducing sugar in three kinds of Fujian Yellow Rice Wine were determined, which were Min Jiang Old Wine, Qing Hong Yellow Rice Wine and Chen Gang Yellow Rice Wine. Folin-ciocalteu method was used to quantify the total polyphenol in yellow rice wine, the linear range of the standard sample Propyl gallate were 0.025～0.600mg/mL(r2=0.9994),the regression equation was Y=2.6325X+0.017. It was the first time to determine the total polyphenol in yellow rice wine with Folin-ciocalteu method, which is simple, fast and accurate.The yellow rice wine samples were isolated by Gel Filtration and reversed-phase high performance liquid chromatography, a peptide RP with anti-ACE activity was isolated and purified. The IC50 was 30.92μg/mL, the molecular weight was around 501Da. According to disc agar diffusion method, RP can inhibit the growth of Physalospora piricola, Fusarium oxysporum and Pythium aphanidermatum. For the first time, the antioxidative activity of Min Jiang Old Wine, Qing Hong Yellow Rice Wine and Chen Gang Yellow Rice Wine was measured by Na2S2O3-I2 titrimetric method, TBA method and DPPH free radical method, respectively. It was found that the three wines could inhibit the lipoperoxidation and the oxidation of TBA; they also had a scavenging effect on the DPPH radical. The DPPH radical scavenging effect of Chen Gang Yellow Rice Wine was especially distinct with a rate of 93.58% at the concentration of 8%. Further studies such as decoloration by activated carbon, HPLC with fluorescence detection were carried out to analyze the antioxidative substances in Chen Gang Yellow Rice Wine. It was found that the fraction M with fluorescent absorbance had strong DPPH radical scavenging activity.