Study On Interkingdom Fusant Between Saccharomyces Cerevisiae And Oenococcus Oeni

The technology of protoplast fusion was beginning on 60's of last century, at present it is already widely used to the domain of zoology and botany and microorganism. This technology may overcome the nature blockade of cell wall, remove the limit of joint, achieve to the multi-gene recombination. Also may break the blood relationship to breed ideal fusant. Thus to accelerate the process of industry microorganism breeding. It has provided a brandy-new way for the fine industry microorganism's selective breeding.This study was aimed at some research of the interkingdom fusant F-20-7 between wine yeast 1450(Saccharomyces cerevisiae) and malo-lactic bacteria SD-2a (Oenococcus oeni), which was come from the college of Enology, N&F University, to prove if it is the interkingdom fusant of the parents. It has done a series of work about latter analysis and identification of fusant.The main studies are as follows:1. According as the experiment of growth, during the fusant growth process, the arrearage period was longer than wine yeast 1450, the logarithm growth period became shorter, and it reached to the stable period with parent wine yeast 1450 almost at the same time.2. After the analyzed of antibiotic, we found the fusant was not sensitive to ampicillin, but sensitive to cycloheximide comparatively, and the bate dose was 100μg/mL. It shows that the descendiblity substance of the fusant was mainly existed in wine yeast.3. According as the experiment of deacidification, the average decomposable efficiency was 68.54%, the tiptop rate was 78.81%. It shows the descendiblity stability of the fusant was not very well.4. The key enzyme gene which induces the malo-lactic fermentation was MleA and MleP. In this study, first we extracted the DNA of nucleolus and did PCR amplification to look for MleA and MleP, but we didn't find it at last. Then we did the same work about the DNA of plasmid and the result was well. Both the MleA and MleP were existed in the DNA of plasmid, and a DNA segment (623bp) of one fusant's plasmid has a highest homology (97%) with gi|55793547|gb|AY786175.1|. By that the...