Synthesis, Characterization And Hydrolysis To Phosphaester-bond Of Amino Acids Complex With Alkaline-Earth Metals

Amino acids constitute a particular important class of difunctional compounds because they are the basic building blocks from which protein or enzymes are construction. Since there are two kinds of functional groups in amino acids, they can coordination via the carboxylate and/or amino groups. Meanwhile, many transitional metal complexes are of interest because of their relevance as active site of biomolecules such as protein and enzymes. So, it is very important to study on the amino acid complexes.By comparation among the hydrolysis effect on PNPPNa2 of the complexes synthesized by several single amino acids and familiar metals, this experiment confirmed that the complex of phenylalanine and magnesium is the optimal complex to hydrolyze phosphaester-bond.A phenylalanine and magnesium complex had been prepared by mixing phenylalanine and MgCO3 in distilled deionized water at 90℃for 8h. The complex was characterized by means of elemental analysis, IR, and XRD. The result showed that phenylalanine as s bidentate ligand coordinated with magnesium ion formed four-coordination complex. The activities of the complex to hydrolyze phosphodiester had also been studied: it can catalyze the hydrolysis ratio of PNPPNa2 to 7.45×10-2min-1(Kobs), and the optimum pH was 8.0, this was most like alkaline phosphatase, but natural alkaline phosphatase cannot hydrolyze phosphodiester; the complex is one of only a few well-defined metal complexes demonstrated to hydrolytically cleave DNA, it can hydrolyze pBR322 DNA at physiological pH and temperature, using rigorously...