Effect Of Pre-storage Temperature On Fruit Calcification And Physiology Of Castanea Mollissima

Chestnut is one of the most important export and distinguishing dry fruits in our country. During the storage, chestnut fruit is very sensitivity to be calcificated, which causes dramatic loss. This thesis carried out a serial of experiments to investigate the effect of pre-storage high temperature on fruit calcification and physiology of Castanea mollissima (var. hongyouli from queshan, Henan province), the correlation between calcification and physiological changes. The main results were as following:1. The higher temperature and longer period treated, the more water loss and more severe calcification in the tissue of chestnut fruits. There were very significant negative relationship between calcification and water content(r=-0.97 p<0.01the same below).With the calcification on-going, respiratory rate , MDA and O₂^- contents were all changed greatly. There were very significant positive correlations between calcification and MDA O₂^- content, respiratory rate ( r=0.95, 0.90, 0.62).2. The higher temperature and longer period treated before storage also caused more water loss and more severe calcification occurred in chestnut during refrigerated storage. Chestnut which had been heat treated accumulated more MDA, and became calcification more easier than CK. There were very significant relationship between calcification and MDA (r=0.78), and positive relationship between calcification and O₂^- and the change rate of respiratory rate (r=0.44, 0.50 p<0.05,the same below). The relationship between calcification and water content reached negative significant level of 0.01 (r=-0.90) .There were significant negative relationship between calcification and amylase activity, POD activity (r=-0.42, -0.51) . Statistical analyses between calcification and relevant indices showed that calcification was mostly related to water content and MDA formation, indicating that water loss and breakdown of cell membrane were the main reasons of chestnut calcification.3. Physiological and biochemical indexes of the chestnut on path analysis were studied by SAS statistic system. The results indicated that water content, amylase and MDA had...