Synthesis And Characterization Of New Nano-luminescent Labels Used In Immunoassay

This paper aims at controllable preparation technology, characterization technology, and biomarker technology of functional lanthanide fluorescence nanoparticles, based on developments of lanthanide luminescent material. Several kinds of lanthanide nanoprecursors were synthesized with gel-network precipitation technique. After calcining these precursors, we get highperformance fluorescent nanocompounds, which are well dispersed and sphere-like with an average diameter of less than 100nm. The luminescent properties were investigated by the fluorescence spectrophotometer. The results show that luminescent features of the products synthesized by the gel-network precipitation technique are similar to those by solid-state reaction, while the calcining temperature is reduced about 200℃-400℃. The conditions of the preparation process were also studied systematically and the optimal conditions were concluded. Then nanophosphors were wrapped with TEOS hydrolyzation, and the nucleocapsid nanoparticles have steady-going luminescence and disperse well.There are six chapters in this magisterial dissertation.In the first chapter, this paper briefly introduces the classification and application of immunoassay and nanophosphor labels, as well as the characteristic and the current development situation of lanthanide fluorescence material.In the second chapter, we used gelatin as the disperser, and successfully prepared Eu³⁺-doped LaPO₄ nanoparticles, which are basically monodisperse, have smaller particle size and better homogeneity. According to our measurements with XRD: the products belong to monoclinic monazite type, and the optimum condition for heat-treatment of the sample was at 600℃ for 2 hours. The emission spectra of LaPO₄: Eu consists of the sharp line emissions in the range of 500～720nm from the transitions of electrons. The excitation spectra have two parts, the wide band at 270nm and the sharp line at 396nm.In the third chapter, Ce,Tb-doped LaPO₄ nanoparticles were successfully...