| Capillary electrophoresis is a powerful technique for the separation and detection in the fields of life science, biological engineering, entironment protect, food detection, medical analysis and so on, due to its virtues of high resolution efficiency, rapid separation, small analyte consumption and simple instrument. Especially the laser-induced fluorescence detection is the most sensitive method, because it can meet to the detection of trace component, single molecule and single cell. However, the laser is restricted to expensive, shorter life, larger bulk, higher power consumption and compounds with matching excitation wavelength. In recently years, Light-emitting-diodes (LEDs) have become very interested in fluorescence detection fields for capillary electrophoresis. As a light resource, LEDs are exceptionally stable, provide high emission intensity, and a wide range of wavelengths from violet to near-Infrared is available. Furthermore, LEDs are small and cheap, provide long lifetime and can be operated with battery power, making them particularly attractive for detector. Currently, the reported detector based on LEDs is not superior in sensitive compare with the laser-induced fluorescence detector, and its application for the separation and detection is few in biological sample.In this work, we developed a novel apparatus with optical fiber light-emitting diode induced fluorescence detection for capillary electrophoresis, and studied on its application in complex biological sample analysis. Firstly, a simple, high sensitive, inexpensive instrument for capillary electrophoresis with optical fiber light-emitting diode fluorescence detection was design and set up in our lab. Then, a series method was developed to detected the trace physiological active substance in biological samples, and was applied to determination D-amino acid and agmitine in biological samples, such as human plasma, human urine, rat brain, rat stomach and so on. The main contents in...
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