Studies On Resonance Scattering And Fluorescence Effects Of Some Dyes Association Particles And Their Analytical Application

Resonance scattering technology and the progress of its application in analytical chemistry in resent years are introduced by inspected the relative materials. The progress of hydroxyl radical and the chlorine oxidation analysis are also introduced. Based on the judgement that the research of the association particles of Rodimine dyes by resonance scattering method and fluorescence method to determine hydroxyl radicals, chlorine dioxide and chlorine are not developed heretoforely. In this paper, we developed resonance scattering method and fluorescence method to determine hydroxy radical, ClO2 and Cl2 by using rhodamie dyes, with satisfactory results. Besides, we develop resonance scattering method to devermine the zine in food, with satisfactory results also. The main aspects and discussion are presented as follows:1. IntroductionResonance scattering technology and the progress of its application in analytical chemistry in resent years are introduced. The progress of hydroxyl radical and the chlorine oxidation analysis are also introduced, including 206 references.2. A Sensitive Fluorescence Method for Determination of Hydroxyl Free Radical in FentonSystem Using Rhodamine S and Its Application in Screening the Antioxidant In HCl-NaAc a buffer solution, hydroxyl radical was produced from Fenton reaction of H2O2-Fe (II), and then it oxidizes excessive I- to form I3-. The four rhodamine dyes including rhodamine B (RhB), butyl rhodamine B (b-RhB), rhodamine 6G (RhG) and rhodamine S (RhS) combine respectively with I3- to lead to fluorescence quenching at 580nm, 550 nm, 550 nm, 580nm respectively. Based on the fluorescence intensity effect of the four systems of RhB, bRhB, RhG and RhS, H2O2 concentration in the range of 0.432~25.9μmol/L, 0.108～13.0μmol/L, 0.216～15.1μmol/L and 0.432～21.6μmol/L can be determined, with a detection limit of 0.39μmol/L, 0.10μmol/L, 0.13μmol/L and 0.40μmol/L, respectively. Therefore, we develop a new fluorescence method to indirect determine hydroxyl radicals. The RhS system is the best for the determination of hydroxyl radicals. It was applied to screening some antioxidant teas, with...