Application Of Molecular Biotechnology About 16S RDNA In Analysis Of Community Structure Of Phosphorus Removal Organism

To investigate the characteristics of the microbial diversity of phosphorus-accumulating organisms in the AN/AO process that are capable of conducting biological phosphorus removal in municipal wastewater treatment, the molecular biological technology based on 16S rDNA has been used such as polymerase chain reaction (PCR), denaturting gradient gel electrophoresis (DGGE), clone of the product of the PCR reaction and so on. On the other hand, the change in microbial community structure depending on the type of electron acceptor, nitrate or oxygen, and on the different pH of each type of electron acceptor was demonstrated by the analysis of the results of 16S ribosomal DNA fragments.With molecular biological methods, the microbial community structure can be analyzed without cultivation by the study of the nuclear molecular (DNA or RNA) in environmental samples. In this work, total DNA was directly extracted from the activated sludge. Work was done to amplify and separate 16S ribosomal DNA fragments of phosphorus removal organism by nested PCR, DGGE, clone and so on. The sequence of several 16S rDNA fragments was determined through comparison with GenBank (NCBI) and some species could be identified. The result showed an overall view of the microbial community structure in the activated sludge of the phosphorus removal process.There are abundant species of phosphorus removal organism existing in the AN/AO process, and the microbial community structure was stable. The microorganism was inevitably flowed by the flow of the sludge, so the bacteria distribution in the reactor was equable. So there were few changes in the community structure of different passages but some species which were probably belong to the group of denitrifying phosphorus removing bacteria (DPB). Nested PCR was used to analyze the community structure of Actinobacteria, Proteobacteria, Acidobacterium by the analysis of the results of denaturing gradient gel electrophoresis of polymerase chain reaction amplified 16S rDNA fragments. And combined with the water quality,...