Breeding Of An Anaerobic Phenol-degradation Strain And Study On Phenolics Biodegradation By The Strain

Phenolics are present in the wastewater of many industries. Phenol, 4-Chlorophenol (4-CP) and m-cresol all are hazardous materials. Even at low concentration, they do harm to biomass for its toxic properties. With the process of economic, wastewater containing phenolics has been greatly poisonous to environment. Biological methods of phenolics removal are preferable in wastewater treatment process as a relatively low processing costs and low possibility of a secondary pollution, because lots of microorganisms can utilize phenolics as carbon resource and energy.In our study, a strain of yeast with high efficient phenolics-degrading bacteria under anaerobic conditions was isolated from acclimated activated sludge, identified as Candida albicans and named as PDY-07.The phenolics degradation was studied under the conditions of different temperature, pH. The results showed that at 35℃,with 150 r/min and sealed, the strain can have pretty high phenol, 4-CP and m-cresol tolerance potential up to 2600 mg/L, 440 mg/L and 1250 mg/L, respectly.In our study, the biodegradation of two substrates contained in the basal medium were studied simply. We just observe the phenomena about the growth of strain and substrates degradation.The kinetics of cell growth and substrates biodegradation of PDY-07 was investigated. Haldane's equation was adopted to describe the growth kinetics of strain during phenol degradation and m-cresol degradation. And 4-CP as sole carbon source and energy for the strain in batch cultures were also investigated using the proposed cell growth kinetic models The degradation kinetic modelμS= AμX+Bwell describes the according substrates degradation behavior.