Study On Phizosphere Microbes Of Bulrush Wetland Of Mengqing Garden

Since long time ago, With increasing population and the industrial development along with the Su Zhou River banks day by day. The massive sanitary sewage and the industrial waste directly disperse into the River having not been disposed. Additional pollution to the the Soviet Zhou River's branches. Causes the pollution load which SuZhou River go far beyond its ability,So quality of the water gradually suffers the pollution. At the end of 70 ' s,the SUZhou River Shanghai sector has been exposed completely the pollution, year to year black and smelly. The Suzhou River environment improvement has also become the most important thing in Shanghai environmental protection and construction. Because the man-made wetland has many merits in the sewage purification, so wo establish the man-made wetland in Meng QiangYuangarden to carry on bioremediation to the Suzhou river water. In the Man-made wetland has the extremely complex chemistry, physical and the biological reaction and the circulation. These reactions are participatied together by zoology and botany and the microorganism in the wetland, Microorganism play the main function in wetland purification sewage. They deal with organic matter as the nutrition and transform them into the material and the energy. Therefore, it has the quite vital significance to investigate the bulrush wetland rhizosphere and the non-rhizosphere.Based on the experiments of Menqing Garden , Analysis property dynamicchange of the bulrush rhizosphere and non- rhizosphere soil. And duplicate a kind of new inoculant , Inspects artificial inoculation to change in the wetland system microorganism's characteristic then to strengthen its handlingability to provide the scientific material. In addition using the PCR-SSCP molecularbiology technology studies the microbial structure change and its the superiority fungus in the differenttimes of the wetland bulrush rhizosphere and in the non-rhizosphere zones. The main conclusions are drawn as followings:1.Long period of monitoring of bulrush unit and reed wetland have shown several results: The number of microorganism in rhizosphere zone is more than non-rhizosphere zone. The quantity of microorganism in three species whatever in both zones can be the same (heterotrophic bacteria > actinomyces> eumycete). The rhizosphere effect of heterotrophic bacteria is most significant. As for nitrifying bacteria the number can be same in rhizosphere and non-rhizosphere (nitrite bacteria> denitrifying bacteria> nitrate bacteria) among which the most significant rhizosphere effect is nitrite bacteria. The intensity of respiration which increases as the quantity growth of microorganism in the soil of rhizosphere zone is higher than non-rhizosphere zone.2 Due to oxygen excretion in the reed rhizosphere zone the Eh which increases as the growth of microorganism quantity in the soil is higher than non-rhizosphere zone. On the contrary, the pH in the rhizosphere zone soil is lower than non-rhizosphere on the account of relative strong absorption of reed to ammonia and nitrogen.3 Experiments with bulrush unit have implied that the quantity of heterotrophic bacteria, actinomyces, eumycete, nitrite bacteria, denitrifying bacteria and nitrate bacteria increases while the detention time is lengthened. The concentration of polluted water can not be over limitation otherwise the quantity of aerobic microorganism will decreases because the oxygen excretion can not satisfy the need of aerobic microorganism.4 Compound microorganism product which was acclaimed by polluted water from Shuzhou creek for 3 weeks could promote the transformation of total organic Nigent and NH₄⁺ -N. The best transformation rate for NH₄⁺ -N is 88.4% after 36h, which is two times than the other microorganism products. The examination of compound microorganism show that species and diversity of compound microorganism product are more than those microorganism products, which validated the method of acclimation is effective.5. The quantity of microbe and activity of enzyme in rhizosphere and non-rhizosphere zones have increased largely after input of inoculants and enzyme, the bacteria in rhizosphere zone. Because of increasing microorganism quantity and the enzyme activity, the experimental is higher than the blank comparison group in contamination removing rate. Regardless of adding the microbial inoculum or not, between the enzyme activity and the quantity of microbe is better, after input of inoculants and enzyme, the relevance achieves above 0.8.6. Compared the quantity of and lower microbes in rhizosphere and non-rhizosphere zones of bulrush wetland, The quantity of the bacteria in upper non-rhizosphere zone is higher than lower, but lower in nitrosomonas, the quantity of the bacteria and nitrobacteria in lower rhizosphere zone is higher than the upper.7. The microbesmass changes biggish with diffent seasons, actinomycetes denitrifying bacteria and nitrifying bacteria changes smooth,but the quantity of the bacteria has a little change in rhizosphere zone. This indicated in the bulrush growth process the root system secretioneach kind of metabolite has provided the nutrition for the microorganism, Even if under the low temperature it also can enable the bulrush rhizosphere to have the very high biomass.8. Studies the main result with the PCR-SSCP technology to the bulrush wetland rhizosphere and microflorastructure is: (1)The rhizosphere microorganism diversity index is higher than the non- rhizosphere, and the rhizosphere microorganism diversity index changes is not big, the non- rhizosphere microorganism diversity index presents the seasonal changes, displays drops in the winter. (2) The microorganism similarity along is lower in non- rhizosphere, but higer in rhizosphere microorganismsimilarity with seasonal variation. The rhizosphere microorganism and the non- rhizosphere microorganism similarity is lower (3)After measures the foreword and the gene bank sequence contrast,the dominant microorganism is Bacillus sp. p10f-4 in non-,but the dominant microorganism is not only Bacillus sp. p10f-4 but also Aeromonas veronii bv. Sobria strain RK 77343 Pseudomonas sp. JN18_A17_R in the rhizosphere, They are playing the vital role processingsewage in the bulrush wetland system.