Preparation And Characterization Of Several Protein Nanomaterials

The technology of preparation of biologic micro/nano materials is the combination of nanotechnology and biologic materials, and the production is the micro/nano materials which has the special physical, chemical and biologic property. It is the foreland and hotspot of the domain of the international biologic technology. It has good application foreground in the medication and sanitation. The nanocarrier of medicine, the biologic nanosensor and the subminiature intelligent medical-treatment instruments will exert important action in the diagnoses and treatment of the diseases and the hygiene. In our study Bovine serum albumin was used as the staple, we gained different nanomaterials just as micro/nano capsules, porous films and nanotubes via template methods.1. The fabrication of Bovine serum albumin hollow micro/nanocapsules.Silica has broad application in biochemistry as inorganic materials, because of that it was stable, it could not be affected by microbe and the change of the pH of the circumstance, and it is synthesized and remove easily. We use silica particles (micron and submicron) as the templates. Bovine serum albumin (BSA) was immobilized on the surface of SiO₂ particles through the process of chemical cross-linking. Finally the templates were removed and the hollow micro/nano capsules were obtained. The multilayer BSA films can be deposited on the surface of the templates by the method of layer-by-layer. So we prepared the microcapsules whose wall was the multilayer BSA films. We discussed how the change of quantity and the concentration of BSA solution and the concentration of cross-linking agent were effected on the thickness of the capsules.2. The synthesize of Bovine serum albumin porous films.The silica particles which have regular sphericity, confined size distribution and be monodisperse were prepared by the method of Vicki Colvin's. Then the silica particles were regularly arranged by self-assembly. After that interspace of the silica films were filled by BSA solution, then BSA were cross-linked and finally the silica particles were removed, the BSA porous films were obtained. The size of the hole can be changed by the changing of the size of silica particles, and the thickness of the films can be changed by the changing of the silica films. 3. The synthesize of the nanotubes of bovine serum albumin and hemoglobin lyophilized bovine erythrocytes.In this study two kinds of porous anodic alumina (AAO) were used as templates (the size of the pore were 120nm and 70-80nm), and bovine serum albumin and hemoglobin lyophilized bovine erythrocytes were used as the staple. At first a monolayer of 3-amino propylphosphonic acid (APA) molecule were attached to the pore wall, The amino groups are then reacted with an excess quantity of the protein-immobilization agent glutaraldehyde (GA), leaving unreacted aldehyde groups on the pore walls, which were react with the amino sites of protein, then the protein was immobilized on the pore wall. The membrane is then alternately exposed to GA and the protein solution. Finally the templates were removed, the protein nanotubes were obtained. The nanotubes were observed by transmission electron microscopy (TEM), the size of the nanotubes was homogeneous and the wall of the nanotubes was slick.