Effects Of Different Metal Ions On The Interaction Between Two Plant Growth Regulators And Bovine Serum Albumin

In recent years, events impacting on the security of food supply have caused wide food scare around the world. Forchlorfenuron has been widely used recently due to the increasing demand for high-quality fruits in the international market But deformed fruit or the shorter storage period were appeared at the same time. And experimental studies have found that health of human will be threatened if they were exposed to forchlorfenuron for a long time Maleic hydrazide is a kind of selective herbicide and temporary plant arowth inhibitor whose active ingredient is potassium salt that enters plant body through stratum corneum of leaf surface to inhibit cell division, reduce plant photosynthesis and inhibit shoot growth However, experimental studies have found that maleic hydrazide resulted in mutation and canceration of plants and animals, which may appear adverse affect after residues flowing into the human body. And as the most abundant proteins in the circulatory system of a wide variety of organisms, serum albumin has many physiological functions. The absorption, distribution, metabolism and excretion of various drugs in plasma can be better understood through quantitative analysis of the interaction between these small molecules and serum albumin. Meanwhile, there are also some important metal ions present in blood plasma, which play important roles in good human health. Therefore, this paper have studied the interaction between plant growth hormones and bovine serum albumin (BSA) by using methods of spectra and molecular docking. The detailed contents are as follows:Firstly, the interaction between forchlorfenuron and bovine serum albumin in the absence and presence of five metal irons was investigated by fluorescence and UV absorption spectroscopy. The binding constants K and binding sites n of forchlorfenuron with BSA in the absence and presence of five metal irons were obtained according to the Stern-Volmer equation. The experimental results showed that a strong fluorescence quenching of BSA was observed and the quenching mechanism was considered as static quenching. The presence of metal ions decreased the binding constants K of forchlorfenuron-BSA complex, following the order of K(Without)> K(Cu2+)> K(Ni2+)> K(Mn2+)> K(Co2+)> K(Zn2+), which indicating that metal ions affect the bind capacity of forchlorfenuron binding to BSA. That means the storage period of forchlorfenuron will be shortened in blood plasma and be cleared quicklySecondly, the interaction between maleic hydrazide and BSA in the absence and presence of five metal irons was investigated by fluorescence and UV absorption spectroscopy. The quenching mechanism of fluorescence of BSA by maleic hydrazide was found to be static quenching, and the binding sites n and apparent binding constant K was calculated through relevant formula. The presence of metal ions also decreased the binding constants of maleic hydrazide with BSA, following the order of K(Without)> K(N2+)> K(Cu2+)> K(Zn2+)> K(Co2)> K(Mn2*), which indicating that metal ions also affect the bind capacity of maleic hydrazide binding to BSA and maleic hydrazide will be cleared quickly.Thirdly, the method of molecular docking was used to study the bonding strength and bonding sites of forchlorfenuron and maleic hydrazide with BSA. The results showed that both of two plant growth hormones could bind with BSA strongly and there was only one binding sites. However, the interaction between forchlorfenuron with BSA was stronger than the interaction of maleic hydrazide with BSA, which was consistent with the conclusion investigated by spectroscotry.