Optimizing Of The Fermentation Process For Acidic Fibroblast Growth Factor

Recent years, Many genetic engineering medicines have been produced successfully by large-scale fermentation of recombinant E. coli. Their genetic characteristics and behavior of physiology also have been understood clearly. However, the expression of different products, the different recombinant bacteria and the different receptors have the different style of training requirements and induced expression. In addition to plasmid instability, but also the existence of the E. coli enzyme hydrolyzing protein and processing problems. So in the process of training often show the instability of the expression level. Therefore, It is of great significance to study the appropriate training conditions for expression of recombinant bacteria and the realize programming production.In this research , the recombinant E. coli human acidic fibroblast growth factor (aFGF) is the study target.High density culture technology and the most suitable medium inoculation are used.Glycerol as the carbon source taking palce of glucose. The carbon source quantity of the medium is regulated by dissolved oxygen and pH changes so that the level of carbon concentration in the lower level. It inhibits acid accumulation, and significantly reduce the acid produced, extending the log. And through a certain amount of nitrogen and magnesium sulfate were added, make the stability of plasmid engineering bacteria has been greatly enhanced.The pilot of the fermentation process improvements to achieve a high density, high yield and concentration training culture, and shorten production cycle. This reduce investment in equipment, so as to achieve lower costs, improve production efficiency. the fermentation process, not the use of special tools, such as oxygen, pressure, optimized the conditions for the project.