The Optimization Of BFGF Fermentation Production Technology

FGF(fibroblast grouth factor) is a kind of cell grouth factor of various styles,coming from mesoderm and neuroectoderm,which has activated biological characters.The known super family of FGF 23 family members so far. Basic Fibroblast Grouth Factor(bFGF)is a member of it,which exists in people and animals'cells commonly,having broad biolofical activated characters.BFGF take functions mainly in promoting various kind of cell division,inducing endothelial cell chemotactic,feeding the neuron,regulating cell metabolism,promoting the formation of new blood vessel and so on.Its value is considerable. However ,the amount of bFGF in human and animal tissues are very low, bFGF, extracted from animal tissue, can not satisfy the research of biological characteristics and clinical applications requirement. In recent years, research on the development and utilization of bFGF is receiving increasing attention while the original production conditions are prostrated. To find a high expression,high yield and low-cost means of production to achieve low-cost engineering production is of great significance .This study is based on recombinant of human basic fibroblast growth factor (bFGF),which takes advantage of high-density culture technique,chooses a suitable culture medium, inoculum concentration , and uses glycerol as carbon source instead of glucose. What is more,it coordinates the amount of carbon source according to the dissolved oxygen and pH changes,which limits the concentration of carbon source at a relatively low level in the culture medium and inhibits the accumulation of a large number of acetic acid. So the production of acetic acid is significantly reduced, and the logarithmic phase is extended. At the same time,choosing the best timing and amount of induction agent, added by a certain amount of nitrogen and magnesium sulfate solution, the stability of plasmid engineered bacteria is greatly enhanced.The study is based on recombinant human basic fibroblast growth factor (bFGF), targeting E. coli, the use of high-density culture technique, choose a suitable culture medium, inoculum size, the use of response surface optimization of medium composition and content. At the same time, the use of response surface method to optimize the culture conditions. Be the last medium and culture conditions. After identification, the expression of biological activity with human basic fibroblast growth factor.In this experiment, we improved the fermentation process to achieve a high density, high yield and high concentration of development, which shortened the production cycle and reduced equipment investment. As a result,the costs was reduced while the production efficiency was increased . Throughout the fermentation process,no use of special instruments were used, such as pure oxygen,pressure, etc.,so that the conditions for engineering were made.