Ultraviolet Detection Of Negative Peaks In High Performance Liquid Chromatography

Compounds that give no or low response on UV, fluorescence and electrochemical detectors are difficult to follow and quantify in normal high-performance liquid chromatography (HPLC) systems. The detection problems can be solved by pre- or post-column derivatization, also can be solved by differential refraction detector and evaporative light scattering detector. But the former is a technically complicated and time-consuming, then the usage of latter suffers to limit. A technically much simpler alternative is used in this research work—ultraviolet detection of negative peaks, an so-call indirect detection method, which is base on the use of a mobile phase with detector response in the chromatography system and can follow and quantify compounds without inherent detector response in high-performance liquid chromatography.IN this research, Sulfosalicylic Acid,1-Naphthol),Niacinamide is applied as background in Reversed-phase high-performance liquid chromatography, which is detectable. A detectable component is included in the mobile phase and is then distributed to the stationary phase. Solutes that are injected in the system give rise to detector response as a result of their influence on the distribution of the detectable component. Three homologues of Acetic ester,seven homologues of Sprout-off,Dexpanthenol are respectively followed and quantified by this method. The factors that affect retention and sensitivity of detection are investigaed ,such as the concertration of background,temperature. The theoretical bachground and properties of the detection system.