Determination Of Polymer Additives Antioxidants And Ultraviolet (UV) Absorbers By High-Performance Liquid Chromatography Coupled With UV Photodiode Array Detection In Food Simulants

An analytical method for the quantitative determination of migrationlevels of polymer additives such as antioxidants and UV absorbers in foodpackages by high-performance liquid chromatography coupled with UV visphotodiode array detection has been developed. The pretreatment stepinvolved solid-phase extraction with silica C18cartridges. The analyticalmethod showed good linearity, presenting regression coefficients (R²)≥0.9990for all compounds. This optimized method was also validated with respect toprecision, reproducibility, stability, and accuracy. The limits of detection andquantification were between0.09and1.72μg mL^-1and between0.20and5.64μg mL^-11for12analytes, respectively. Recoveries were in the range of67.48and108.6%, with relative standard deviations between2.76and9.81%.Migration levels of antioxidants and UV absorbers were determined. Butylated hydroxyanisole(BHA),2,6-di-tert-butyl-4-methylphenol(BHT),2,4-di-tertbutylphenol(DBP), Cyanox2246, Irganox1035, Tinuvin326, Tinuvin328, Irganox1010, and Irganox1330were detected, BHT and Cyanox2246were at higherlevels than the specific migration levels in some food simulants.