Study On The Production Technics Of 6-aminopenicillanic Acid (6-APA)

6-APA is widely used as the material of semi-synthetic penicillin. It is usually prepared from solid penGK through enzymatic hydrolysis. The process is known to be costly and the yield is low. The use of organic solvent may cause environmental problems. Efforts are made to look for new ways to produce 6-APA. In the thesis, we propose a Direct Process that prepare 6-APA directly from the K2CO3 solution of penGK (RB solution).The yield may increase 10% with the deletion of the crystallization unit in the new process. However, much impurity remains in the RB solution without the crystallization may affect the quality and the yield of 6-APA. The feasibility analysis indicates that the BuoAc in the RB solution may affect the activity of the enzyme and the transmittance of the RB solution is the key factor which influence the quality of 6-APA. Experiments are done in the study to optimize the whole production procedure. (1) In the extraction unit, the pH value and the phases ratio are optimized to 2.5 and 2:1, respectively. The ratio of BA and water is adjusted to 2:1 in the washing unit. (2) The Freezing Unit and the Decolouring Unit are introduced into the process to control the color grade of the BA solution and the quality of the product. (3) The concentration of the K₂CO₃ solution is adjusted to 3.5% in the ob- extraction unit. (4) The Decompressing Azeotropic Distillation Unit is adopted to reduce the quantity of BuoAc in the RB solution. With the methods listed above, the total extraction yield of more than 89.3% is achieved. The quality and the yield of product in the new process are the same as that in the old one but the cost is much lower than before.