Preparation And Analysis-Application Of Terbium Complexes With Nanosized Silicon Dioxide

The core-shell fluorescent nanoparticles was prepared in a water-in-oil (W/O) microemulsion containing Triton X-100, hexanol and cyclohexane by controlling copolymerization of tetraethyl orthosilicate and 3-aminopropyl-triethyoxysilane. The nanoparticles are spherical and uniform in size, about 50nm in diameter, strongly fluorescent, and highly stable. Especially, the method significantly reduces leaching of fluorescent compounds from the nanoparticles, and are promising as efficient biological labels.Secondly, on the base of this, The superparamagnetic Fe₃O₄ or SiO₂ particle is prepared. And the -NH₂ group is modified on the Fe₃O₄ or SiO₂ particle surface via silanization by reagent. The modified nanoparticles have good chelation with terbium. Especially, the nanoparticles which wrapped with nanosized silicon dioxide, solution has little effect on this compound after terbium get in this functional nanoparticles of complex structure. It can prevent the compound separation. So, It is very useful in separation and preconcentration of terbium. The detection limit for Tb³⁺ was 1×10^-9mol/L.