The Fluorescent Research And Immunoassay Of Dye And Compound Doped Silica Nanoparticles

Luminescent nanoparticles have become an attractive area of research in recent years; it has been known that they can be used as luminescence probes for various bioassays. Compared to conventional organic fluorescence probes, advantages of the nano-sized luminescence probes mainly include their higher photostability and stronger luminescence, which allows them favorably to be used as luminescence probes for bioassays. Nanoparticles are usually prepared into core-shell nanoparticles in order to modified them easily, While silica nanoparticles is one of them that have been widely used, because they have shown several advantages in high water-solubility, easy preparation, separation and surface modification. But the problem of dye leakage from the silica matrix after the dispersion in an aqueous solution still existed if there are no retaining forces between dye molecules or lanthanide chelates and the silica matrix; what's more, luminescence efficiency is greatly affected by fluorescence quenching in solution.In this paper, a serial of fluorescent nanoparticles without dye leakage are prepared in the effect of chemical bond, they are very uniform with a diameter between 30 and 50nm. The fluorophores doped inside the nanoparticles are well protected from the surrounding environments and, thus, the potential quenching and bleaching in solution are decreased. A novel fluorescence labeling technique was established based on fluorescence nanoparticles and integrated with nanotechnology, biotechnology and fluorescence labeling technique.In chapter two, a monomer silica precursor as core material was obtained by coupling dansyl-Cl (DNS-Cl) with 3-aminopropyltriethyloxysilane (APTEOS); we have successfully prepared fluorescent hybrid silica nanoparticles via water-in-oil (W/O) microemulsion method. Owing to a firm chemical bond between DNS-Cl and APTEOS, dye leaching from the nanoparticles is significantly reduced, but dye of sulfonyl chloride is few, which limits their application. In chapter three, a precursor was prepared by coupling fluorescein with DTPAA and APTEOS; a fluorescein doped...